BS IS0 13304-1:2020 BSl Standards Publication Radiological protection Minimum criteria for electron paramagnetic resonance (EPR) spectroscopy for retrospective dosimetry of ionizing radiation Part 1: General principles bsi. BS ISO 13304-1:2020 BRITISH STANDARD National foreword This British Standard is the UK implementation of ISQ 13304-1:202Q The UK participation in its preparation was entrusted to Technical Committee NCE/2, Radiation protection and measurement. A list of organizations represented on this committee can be obtained on request to its committee manager. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. @ The British Standards Institution 2020 Published by BSI Standards Limited 2020 ISBN978 0539 131437 ICS 17.240; 13.280 Compliance with a British Standard cannot confer immunity from legal obligations. This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2020. Amendments/corrigenda issued since publication Date Text affected BS IS0 13304-1:2020 INTERNATIONAL ISO STANDARD 13304-1 Second edition 2020-07-20 Radiological protection Minimum criteria for electron paramagnetic resonance (EPR) spectroscopy for retrospective dosimetry of ionizing radiation - Part 1: General principles Radioprotection - Criteres minimaux pour la spectroscopie par résonance paramagnétique électronique (RPE) pour la dosimétrie rétrospective des rayonnements ionisants - Partie 1: Principes généraux Reference number ISO 13304-1:2020(E) ISO @IS02020 BS IS0 13304-1:2020 IS0 13304-1:2020(E) COPYRIGHTPROTECTED DOCUMENT IS0 2020, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission.Permission can be requested from either Iso at the address below or Iso's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org ii @IsO2020-Allrightsreserved BS IS0 13304-1:2020 IS0 13304-1:2020 Contents Page Foreword .iv Introduction. V 1 Scope. 2 Normative references 1 3 Terms and definitions 1 4 Confidentiality and ethical considerations .2 5 Laboratory safety requirements .3 5.1 Magnetic field. 3 Electromagnetic frequency 5.2 5.2.1 invitro measurement 3 5.2.2 3 in vivo measurement 5.3 Biohazards from samples .3 6 Collection/selection and identification of samples .3 7 Transportation and storage of samples ..4 8 Preparation of samples. .4 .5 9 Apparatus. Principles of EPR spectroscopy 9.1 9.2 Requirements for EPR spectrometers .6 9.3 .6 Requirements for the resonator. 9.4 Measurements of the background signals. 6 9.5 Spectrometer stability and monitoring/control of environmental conditions .6 9.6 7 Baseline drift 10 Measurements of the samples 7 10.1 General principles. 7 10.2 Choice and optimization of the measurement parameters 7 10.2.1 General 7 10.2.2 Microwave-related parameters .8 10.2.3 Magnetic field parameters. .8 10.2.4 Signal channel parameters .8 10.3 Sample positioning and loading 9 10.4 Microwave bridge tuning. .10 10.5 Use of standard samples as field markers and amplitude monitors .10 10.6 Monitoring reproducibility. 10 10.7 Procedure to measure anisotropic samples .10 10.8 Coding of spectra and samples.. .11 Determination of the absorbed dose in the samples. 11 .11 11.1 Determination of the radiation-induced signal intensity .11 11.2 Conversion of the EPR signal into an estimate of absorbed dose .11 11.2.1 Conversion of the EPR signal into an estimate of absorbed dose for in 11 vitro dosimetry 11.2.2 Conversion of the EPR signal into an estimate of absorbed dose for in vivo tooth dosimetry .12 12 Measurement uncertainty ..12 13 Investigation of dose that has been questioned ..12 14 Quality assurance (QA) and quality control (QC) ..13