International ISO Standard ISO7218 Fourth edition Microbiology of the food chain- 2024-06 General requirements and guidance for microbiological examinations Microbiologie de la chaine alimentaire -Exigences générales et recommandations pour les examens microbiologiques Reference number ISO 7218:2024(en) O ISO 2024 ISO 7218:2024(en) COPYRIGHT PROTECTED DOCUMENT O ISO 2024 All rights reserved.Unless otherwise specified,or required in the contextof its implementation,no part of this publication may the internet or an intranet,without prior written permission.Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office CP 401:Ch.de Blandonnet 8 CH-1214Vernier,Geneva Phone:+41227490111 Email:[email protected] Website:www.iso.org Published in Switzerland OISO 2024-All rightsreserved ii 7218:2024(en) ISO Contents Page Foreword vii Introduction vii 1 Scope 2 Normative references 3 Terms and definitions Premises 4 4.1 General 4.2 Biosafety considerations 4.3 Laboratory design 4.4 Laboratory areas 4.4.1 General 4.4.2 Areas associated with samples and testing 4.4.3 General areas 6 4.5 Layout and fittings ofthe premises 4.5.1 Objectives Fittings 4.5.2 4.5.3 Other arrangements for laboratory premises 4.5.4 Cleaning and disinfection 5 Personnel 5.1 General 5.2 Competence 5.3 Verification ofongoing staff competence 5.4 Hygiene Equipment and consumables 6 6.1 General Sterilization and otherheating 6.2 equipment 10 General 10 6.2.1 10 6.2.2 Autoclave Culture media preparator 6.2.3 6.2.4 Steamers , including boiling -water baths 12 6.2.5 Sterilizing oven 6.2.6 Microwave oven 13 6.2.7 Hotplate , induction cooker and heating mantle 14 Gas burner or wire incinerator 6.2.8 6.3 Temperature controlled equipment and monitoring devices 15 6.3.1 General .15 6.3.2 Incubator 15 6.3.3 Thermostatically controlled bath 16 ..... 6.3.4 blocks Heating 17 6.3.5 Refrigerators and cold -storage rooms 18 6.3.6 Freezer and deep i freezer /ultra -low temperature freezer 19 6.3.7 -monitoringdevices 19 Temperature ,including automatic recorders Balances and gravimetric 6.3.8 diluters 20 Defined volume inoculation equipment 6.4 21 6.4.1 Pipettes 21 and pipettors Dispensers 6.4.2 2 6.4.3 23 Spiral platers Serial diluters 6.4.4 24 6.5 Protective cabinets 24 6.5.1 24 Description 6.5.2 25 6.5.3 Cleaning 25 and disinfection Maintenance 6.5.4 and inspection 26 @ ISO 2024-Allrights reserved ii